Producing homogenous and conductive metal or carbon coatings for SEM and TEM analysis was never more convenient than with the Leica EM ACE200 coating system. Configured as a sputter coater or carbon thread evaporation coater, perfectly reproducible results can be achieved in the fully automated system. If both methods are required for your analysis Leica Microsystems offers a combined instrument with interchangeable heads in one instrument. Various options as Quartz Crystal Measurement, Planetary Rotation, Glow Discharge, and Exchangeable Shielding complete this Low Vacuum Coater.

With Leica EM VCT500 you can transfer samples under cryogenic conditions or at room temperature as well as under vacuum or protected atmosphere.

The Leica TCS SPE is an affordable entry to the Leica family of confocals. It combines a straightforward design with high-end, true point-scanning confocal technology. The prism-based spectral detection – unique to all Leica confocal microscopes – and a highly dynamic photomultiplier offer extraordinary signal efficiency for gapless detection of even weak signals. A variety of robust solid-state lasers allow the use of a broad range of common dyes. The Leica TCS SPE confocal is a true point-scanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells. With its small footprint , the Leica TCS fits in every laboratory at common room conditions. The Leica TCS SPE is the only confocal in its class that provides true spectral detection suitable for lambda scan.

Products – Page 23 – DMI Medical USA

Q: Leica EM CDT | Cryo Tool Drying

During Cryo preparation, tools such as forceps and sample holders often ice over and cannot be immediately used for the next sample run. De-icing and drying of the tools at room temperature is time consuming and can cause damage to sensitive components and may prevent their operation. The special combination of heated air flow and heating plate completely dries tools, even on their difficult to access inner surfaces. The maximum temperature of + 50°C ensures that the cryo tools dry without heat damage and protect the user from the risk of burns

Q: Leica EM AFS2 and EM FSP

Freeze Substitution and Low Temperature Embedding System The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use Reduces set-up time Automatic dilution of reagents from 100% stock containers reduces user reagent handling and set-up time. Specimens remain dry Adjustable transfer function of gaseous LN2 allows the chamber and specimens to remain dry, which results in no contamination of the specimen One Step preparation Fast UV polymerization with LED UV lamp results in one-step preparation for the user; samples and reagents can be added and left until the end of the run –140 °C to +70 °C working range AFS smart-remote observation of the process and delivery of critical information via SMS Stereomicroscope viewing

Q: Leica EM GP2 | Automatic Plunge Freezer

The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting. Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %. Safe and reproducible preparation for single particle analysis and cryo-tomography.

Q: Leica EM ICE | High Pressure Freezer

High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics. Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process. Capture and resolve highly dynamic processes at the nanometer scale with millisecond precision. The EM ICE solution, combining superior high pressure freezing with the possibility of light and electrical stimulation, is the platform for your discoveries LN 2 Consumption Value LN 2 consumption during cool down 80lts 8 liters LN 2 consumption in operation including cool down 30lts 30 liters LN 2 consumption per freezing process 80ml 80 ml

Q: THUNDER Imager EM Cryo CLEM

The THUNDER Imager EM Cryo CLEM is a cryo light microscope featuring opto-digital THUNDER technology. It provides the imaging data and secure cryo conditions you need for successful experimental investigations concerning structural biology. Precisely identify cellular structures of interest thanks to high resolution, haze-free imaging with THUNDER technology, then transfer the specimen seamlessly to your EM. Benefits include: Fast, high-resolution imaging and elimination of out-of-focus blur with THUNDER technology Precise, seamless transfer of image data to different EM solutions Optimal cryo conditions maintained throughout the imaging workflow and sample transfer EM Cryo CLEM combines a high-resolution cryo objective with THUNDER technology from Leica Microsystems. The result is crisp, haze-free images. The system ensures contamination free sample transfer and loading from cryo sample preparation instruments to Leica fix stage light microscope. Maintains sample vitrified during cryoimaging.

Q: Leica EM VCM | Vacuum Cryo Manipulation

The Leica EM VCM loading station enables you to connect different preparation techniques to various analysis devices such as Cryo-SEM, Cryo-TEM and Cryo-CLEM

Q: Array Tomography Solution ARTOS 3D

The ARTOS 3D (ARray TOmography Solution) automatically creates and collects hundreds of serial-sections ribbons all ready for array tomography with your scanning electron microscope (SEM). Save time and effort in biological sample preparation and SEM setup procedure so you can quickly get the images you need to answer critical research questions.

Q: Cryochamber Leica EM FC7

Low temperature ultrathin cryosectioning of biological and industrial samples. Can be mounted on the EM UC7 and the ARTOS 3D. The EM FC7 outfitted with an EM VCT500 transfer port is the ideal solution for environmentally sensitive sample and / or cryogenic sample transfer.