CRYO PREPARATION – DMI Medical USA

Q: Leica EM CDT | Cryo Tool Drying

During Cryo preparation, tools such as forceps and sample holders often ice over and cannot be immediately used for the next sample run. De-icing and drying of the tools at room temperature is time consuming and can cause damage to sensitive components and may prevent their operation. The special combination of heated air flow and heating plate completely dries tools, even on their difficult to access inner surfaces. The maximum temperature of + 50°C ensures that the cryo tools dry without heat damage and protect the user from the risk of burns

Q: Leica EM AFS2 and EM FSP

Freeze Substitution and Low Temperature Embedding System The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use Reduces set-up time Automatic dilution of reagents from 100% stock containers reduces user reagent handling and set-up time. Specimens remain dry Adjustable transfer function of gaseous LN2 allows the chamber and specimens to remain dry, which results in no contamination of the specimen One Step preparation Fast UV polymerization with LED UV lamp results in one-step preparation for the user; samples and reagents can be added and left until the end of the run –140 °C to +70 °C working range AFS smart-remote observation of the process and delivery of critical information via SMS Stereomicroscope viewing

Q: Leica EM GP2 | Automatic Plunge Freezer

The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting. Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %. Safe and reproducible preparation for single particle analysis and cryo-tomography.

Q: Leica EM ICE | High Pressure Freezer

High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics. Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process. Capture and resolve highly dynamic processes at the nanometer scale with millisecond precision. The EM ICE solution, combining superior high pressure freezing with the possibility of light and electrical stimulation, is the platform for your discoveries LN 2 Consumption Value LN 2 consumption during cool down 80lts 8 liters LN 2 consumption in operation including cool down 30lts 30 liters LN 2 consumption per freezing process 80ml 80 ml