With Leica EM VCT500 you can transfer samples under cryogenic conditions or at room temperature as well as under vacuum or protected atmosphere.

EM Samp Pr – DMI Medical USA

Q: Leica EM ICE | High Pressure Freezer

High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics. Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process. Capture and resolve highly dynamic processes at the nanometer scale with millisecond precision. The EM ICE solution, combining superior high pressure freezing with the possibility of light and electrical stimulation, is the platform for your discoveries LN 2 Consumption Value LN 2 consumption during cool down 80lts 8 liters LN 2 consumption in operation including cool down 30lts 30 liters LN 2 consumption per freezing process 80ml 80 ml

Q: Leica EM GP2 | Automatic Plunge Freezer

The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting. Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %. Safe and reproducible preparation for single particle analysis and cryo-tomography.

Q: Leica EM AFS2 and EM FSP

Freeze Substitution and Low Temperature Embedding System The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use Reduces set-up time Automatic dilution of reagents from 100% stock containers reduces user reagent handling and set-up time. Specimens remain dry Adjustable transfer function of gaseous LN2 allows the chamber and specimens to remain dry, which results in no contamination of the specimen One Step preparation Fast UV polymerization with LED UV lamp results in one-step preparation for the user; samples and reagents can be added and left until the end of the run –140 °C to +70 °C working range AFS smart-remote observation of the process and delivery of critical information via SMS Stereomicroscope viewing

Q: Leica EM CDT | Cryo Tool Drying

During Cryo preparation, tools such as forceps and sample holders often ice over and cannot be immediately used for the next sample run. De-icing and drying of the tools at room temperature is time consuming and can cause damage to sensitive components and may prevent their operation. The special combination of heated air flow and heating plate completely dries tools, even on their difficult to access inner surfaces. The maximum temperature of + 50°C ensures that the cryo tools dry without heat damage and protect the user from the risk of burns

Q: Leica EM ACE900 | Freeze Fracturing System

The Leica EM ACE900 is a high-end sample preparation system for freeze fracturing, freeze etching and high resolution cryo coating. This instrument is easy and intuitive to use. To achieve highest sample protection sample transfer, large cryo-shields, and the sample shutter are optimized. Users obtain best results because the instrument stays always in high vacuum. This is provided by a load lock system, a knife transfer, and gate valves for each e-beam source. The perfect combination of automation and optional manual operation makes the preparation process highly reproducible and flexible. Versatility, speed, and yield of your sample preparation is incomparable

Q: Leica EM TXP | Target Surfacing System

The Leica EM TXP is a target preparation device for milling, sawing, grinding, and polishing samples prior to examination by SEM, TEM, and LM techniques. An integrated stereomicroscope allows pinpointing and easy preparation of barely visible targets. With the specimen pivot arm the sample can be observed directly at an angle between 0° and 60°, or 90° to the front face for distance determination with an eyepiece graticule

Q: Leica EM Rapid | Pharmaceutical Milling System for Pills

The Leica EM RAPID milling system for pharmaceutical research enables sample preparation for analysis of active ingredient distribution on solid-dose pharmaceuticals. The instrument, using a tungsten carbide or diamond miller, decapsulates pills without smearing and features adjustable cutting speeds between 300 and 20,000 rpm, defined step-layer removal, low-noise extraction, and filtration with a Hepa filter and single or multiple holders.

Q: Leica EM TRIM2 | Specimen Trimming Device for SEM TEM & LM

The Leica EM TRIM2 is a high-speed milling system with integrated stereomicroscope and LED ring illuminator for trimming of biological and industrial samples prior to ultramicrotomy. The Leica TRIM2 features a 1µm feed control for the milling cutter, perpendicular viewing of the embedded sample to determine position for accurate milling, and a superior extraction system with Hepa filter.

Q: Leica EM TIC 3X | Ion Beam Milling System

The Triple Ion Beam Milling System, EM TIC 3X allows production of cross sections and planar surfaces for Scanning Electron Microscopy (SEM), Microstructure Analysis (EDS, WDS, Auger, EBSD) and AFM investigations. With the EM TIC 3X you achieve high quality surfaces of almost any material at room temperature or cryo, revealing the internal structures of the sample in a near native state as possible. the Leica EM TIC 3X can be configured for applications of standard preparation, high throughput processing, and the preparation of extremely heat sensitive samples such as polymers, rubbers, or even biological material at low temperatures. Samples can be transferred to the (cryo) SEM under cryo-vacuum condition